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Test methods evaluated by JaCVAM for regulatory uses

Test methods endorsed by JaCVAM

No. Test methods Years and months
1 Vitrolife-SkinTM, a 3-dimensional cultured skin model for skin corrosivity testing Aug. 2008
2 LLNA-DA for skin sensitization testing Nov. 2008
3 In vitro ocular toxicity test methods for identifying ocular corrosive and severe irritations: Isolated Chicken Eye (ICE) test method Dec. 2009
4 In vitro ocular toxicity test methods for identifying ocular corrosive and severe irritants: Bovine Corneal Opacity and Permeability (BCOP)test method Dec. 2009
5 In vitro assays using the reconstructed human epidermis model, EPISKIN for skin irritation testing Mar. 2010
6 LLNA-BrdU-ELISA for skin sensitization testing May. 2010
7 Cytotoxicity tests to estimate starting doses for acute oral systemic toxicity tests Jun. 2011
8 In vitro assays using the reconstructed human epidermis model, EpiDerm and SkinEthics for skin irritation testing Jan. 2013
9 Local Lymph Node Assay(LLNA):DA for skin sensitization assay Jan. 2013
10 Local Lymph Node Assay(LLNA):BrdU-ELISA for skin sensitization assay Jan. 2013
11 Reduced Local Lymph Node Assay(rLLNA) for skin sensitization assay Jan. 2013
12 Fluorescein Leakage (FL) test method for identifying ocular corrosives and severe irritants Jan. 2013
13 In vitro assays using the reconstructed human epidermis models, LabCyte EPI-MODEL24 for skin irritation testing Nov. 2013
14 Bovine Corneal Opacity and Permeability (BCOP) test method for identifying
i) Chemicals inducing serious eye damage and ii) Chemicals not requiring
classification for eye irritation or serious eye damage
Jan. 2014
15 Revised Test Guideline No. 405: Acute eye irritation/corrosion Jan. 2014
16 BG1Luc estrogen receptor transactivation test method for identifying
Estrogen Receptor (ER) agonists and antagonists
Jan. 2014
17 Skin absorption in vitro method Jan. 2014
18 Isolated Chicken Eye (ICE)test method for identifying i) Chemicals inducing serious eye damage and ii) Chemicals not requiring classification for eye irritation or Sserious eye damage Jan. 2015
19 Direct Peptide Reactivity Assay (DPRA) for skin sensitisation testing Mar. 2015
20 ARE-Nrf2 Luciferase (KeratinoSens) test method assay for skin sensitisation testing Aug. 2015
21 Reactive Oxygen Species (ROS) assay for photosafety assessment Jan. 2016
22 Short Time Exposure (STE) In Vitro test method for assessing ocular irritation Mar. 2016
23 Stably Transfected Transcriptional Activation (STTA) assay to detect Estrogen Receptor (ER) mediated activity Dec. 2016
24 Reconstructed human Cornea-like Epithelium (RhCE) eye irritation test method Jan. 2017
25 human Cell Line Activation Test (h-CLAT) for skin sensitization test method Mar. 2017
26 In vitro skin corrosion: Reconstructed Human Epidermis (RHE) test method Jun. 2017
27 In vitro skin corrosion: Transcutaneous Electrical Resistance (TER) test method
Oct. 2017
28 In vitro membrane barrier test method for skin corrosion Oct. 2017
29 RhCE test method, SkinEthic™ HCE EIT Mar. 2018
30 U937 Cell Line Activation Test (U-SENS™) Skin Sensitization Test Method Nov. 2018
31 Stably Transfected human androgen receptor Transcriptional Activation (STTA) assay for detection of androgenic agonist and antagonist activity of chemicals using the AR-EcoScreenTM cell line Feb. 2019
32 LabCyte CORNEA-MODEL24 eye irritation test for assessing ocular irritation Feb. 2019
33 In vitro cytotoxicity assay for estimating acute oral toxicity Apr. 2019
34 2018 revision of OECD Test Guideline 438: Isolated Chicken Eye (ICE) test method for eye irritation or serious eye damage Nov. 2019
35 ARE-Nrf2 luciferase (LuSens) test method for skin sensitization Nov. 2019

Test methods not endorsed by JaCVAM

No. Test methods Years and months
1 Yeast-RBC (Red Blood Cell) assay Jan. 2010
2 In vitro pyrogenecity assay Dec. 2010
3 Syrian hamster embryo cell transformation assay Mar. 2018